WebNational Center for Biotechnology Information http://www.immunohistochemistry.us/IHC-protocol/paraffin-section-protocol.html

Comprehensive comparison and applications of different sections …

http://mousepheno.ucsd.edu/histo.shtml WebProcedure for deparaffinization of paraffin-embedded sections before staining. Print this protocol. Before proceeding with the IHC staining protocol, the slides must be deparaffinized and rehydrated. Incomplete removal of paraffin can lead to poor staining of the section. Materials and reagents Xylene 100% ethanol 95% ethanol 70% ethanol on the forefront of your mind

Immunofluorescence for paraffin-embedded tissue sections

WebA. Sectioning Protocol: Section paraffin blocks at the desired thickness (usually 4-5 µm) on a microtome and float on a 40°C water bath containing distilled water. Transfer the sections onto a Superfrost Plus slide. Allow the slides to dry overnight and store slides at room temperature until ready for use. WebHonestly paraffin sections are much easier to work with during the staining/handling procedures than frozen. Depending on the tissues, frozen sections are extremely hard to cut and attach to... WebJan 1, 2013 · In parallel, the most commonly used sectioning done today is paraffin sectioning, cryostat, and vibratome cutting. Vibratome sections can be used in both LM and EM studies, but vibratome sections are mostly used for pre-embedding histochemical or immunohistochemical stains in EM studies. ions negative and positive