WebIn this study, we show that a modified vector architecture combined with the introduction of new tet-responsive promoters, Ptet, improved the dynamic range of such all-in-one vectors to levels up to 14,000-fold for viral and 25,000-fold for nonviral transfer vectors in nonclonal human cell lines, and up to 2,800-fold in murine hematopoietic ... WebIn this study, we show that a modified vector architecture combined with the introduction of new tet-responsive promoters, Ptet, improved the dynamic range of such all-in-one …
METHODOLOGY ARTICLE Open Access Improved Tet-responsive …
WebMay 22, 2024 · The relative expression of a chromosomal copy of mstA from its native promoter or Ptet-mstA in exponentially grown WT and mutant cells as measured by qRT-PCR. Values are means ± SD from three independent experiments. ... Moreover, this induction is strictly dependent of 3MST activity: deletion of mstA abolishes tcyP … WebJan 25, 2024 · In addition, the Ptet promoter was also replaced with another constitutive strong promoter, Peno, with integration at the ZMO0038 chromosomal locus to generate ZMQ 5 (Fig. 6a). The titers of isobutanol production in ZMQ3, ZMQ4, and ZMQ5 were 144.33 ± 7.23, 147.33 ± 1.15, and 120.00 ± 3.00 mg/L under the induction of 1 μg/mL tetracycline … cii rap sheet definition
Tetracycline inducible expression—Tet-One systems
Web16 rows · The initial system Gossen and Bujard developed is known as … WebJul 1, 2024 · Subsequent mutations of the minimal CMV moiety were made and these mutants were tested for background and induced activity. The sixth promoter mutant in this series, pTet-T6, proved the most effective, vastly outperforming both pTet-1 and pTet-14 in fold change induction between background and induced states [49]. Web2000). The inducible promoter, P. Tight, provides for very low basal expression and tightly controlled induction. • The Tet-Off Advanced transactivator. The pTet-Off Advanced vector constitutively expresses the tetracy-cline-controlled transcriptional transactivator, Tet-Off Advanced (Urlinger . et al., 2000). This engineered protein consists ... cii regulation shipping